Performance Protein Solutions
Powered by Bota Freeway™, Bota Bio offers customizable high-performance protein solutions with efficient iteration, screening the best proteins and strains from our vast, proprietary datasets to increase protein yield at reduced cost.
Challenges
Protein expression is limited by multiple bottlenecks, such as transcription efficiency, translation efficiency, metabolic constraints, protein folding, and hydrolysis. Additionally, effectively increasing protein yield while meeting cost targets remains a significant challenge.
Our Solution
By screening a library of 95 signal peptides, we enhanced protein entry into the secretory pathway, achieving a 2 to 4-fold increase in expression levels. Using multi-copy gene integration technology, up to 12 gene copies can be integrated in a single transformation, resulting in up to a 9-fold increase in yield. Potential bottlenecks are addressed through over-expression and knockout library screening, with each round of screening potentially doubling the yield.
95
Our signal peptide library contains 95 natural, hybrid, or synthetic signal peptides, increasing expression levels by 2 to 4 times.
12
Up to 12 gene copies can be integrated during one transformation reaction, resulting in up to 9-fold titer improvement.
80%
Fermentation Performance Reproducibility
Results and Impact
Through multiple rounds of strain engineering and screening, protein expression has significantly improved, with strain performance and fermentation process reproducibility exceeding 80%. The optimized production process has significantly reduced costs while enhancing product quality and purity, making commercial-scale production feasible.
Our Performance Protein Products
You can learn more about our products at the official website of Yucca Elements, our personal care brand:
Prorylia™ Product
Fibronectin
